Effect of Protease Treatment of Fixed Platelets on Their Capacity to Enhance Aggregation
نویسنده
چکیده
The role of fibrinogen in interplatelet recognition during aggregation was examined by combining two cell types: fresh platelets (in limiting density) activated by thrombin or A231 87. and formaldehyde-fixed platelets. bearing crosslinked fibrinogen. The fixed platelets did not aggregate by themselves. nor with resting platelets, but were capable of interacting with activated platelets and of participating passively in aggregation. The participation. expressed by enhanced aggregation. was assayed by the conventional turbidometric traces and by cosedimentation of fixed 3Hplatelets with aggregates of fresh platelets. Platelet suspensions. prepared without special means to avert spontaneous activation. retained plasma fibrinogen to the extent of 50 .tg/ml of a suspension containing i0 platelets. and
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